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. 2012 Apr 4;32(14):5002–5009. doi: 10.1523/JNEUROSCI.1411-11.2012

Figure 1.

Figure 1.

BDNF mRNA is not present in cells of the transgenic knock-outs used as nerve graft donors. A, Based on results of FISH, BDNF mRNA is present in the cells in the ventral horn of the spinal cord of untreated wild-type mice. B, Two weeks of treadmill training (TT) resulted in increased labeling of BDNF mRNA-positive cells in wild-type mice. C, No BDNF mRNA was detected in tissues collected from tam-Cre::BDNFf/f mice, regardless of training condition. D, BDNF allele deletion for the CNTF-Cre::BDNFf/f mice was assessed using a Cre reporter mouse, termed tomato. All cells in the sciatic nerve of the tomato mouse are tomato fluorophore-positive. E, After expression of Cre-recombinase, Cre+ cells express green fluorescent protein. The presence of green fluorescence in presumptive Schwann cells indicates that Cre recombinase is active in cells of the sciatic nerve of CNTF-Cre-expressing mice. These data support the idea that Cre activity in CNTF+ cells in CNTF-Cre::BDNFf/f mice removes BDNF from CNTF+ cells.