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. 2012 Jun 22;7(6):e39557. doi: 10.1371/journal.pone.0039557

Figure 1. Genetic transformation of banana cv. Rasthali with full-length Petunia defensin constructs.

Figure 1

(A) Petunia flower which served as the source of defensin genes. (B) T- DNA region of pPhDef1-1301/pPhDef2-1301 binary vector wherein PhDef1 and PhDef2 coding sequences were cloned downstream of Zea mays polyubiquitin promoter and upstream of nos (nopaline synthase) 3′ UTR in MCS of pCAMBIA-1301 vector. (C) and (D) Somatic embryos derived from pPhDef1-1301 and pPhDef2-1301 transformed cells on embryo induction medium 6 weeks post cocultivation. (E) and (F) Germination of transgenic somatic embryos derived from pPhDef1-1301 and pPhDef2-1301 transformed cells on medium supplemented with BAP 10 weeks post cocultivation. (G) and (H) Multiple shoots derived from pPhDef1-1301 and pPhDef2-1301 transformed cells on shoot induction medium 14 weeks post cocultivation. (I) and (J) Individual shoots derived from pPhDef1-1301 and pPhDef2-1301 transformed cells on root induction medium supplemented with NAA. (K) Hardening of rooted transgenic plants in the green house.