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. 2012 Jun 22;7(6):e39557. doi: 10.1371/journal.pone.0039557

Figure 8. Expression analyses of the transgenic banana plants.

Figure 8

(A) and (B) RT-PCR of selected pPhDef1-1301 and pPhDef2-1301transgenic lines performed using primers specific to PhDef1 and PhDef2. (C) and (D) Northern blot analysis of selected pPhDef1-1301 and pPhDef2-1301 transgenic lines performed using FA-MOPS gel. Total RNA isolated from the transgenic leaves was filter hybridized with DIG labeled DNA probes targeted against PhDef1 (C) and PhDef2 (D) gene. Note the high-level expression of Petunia defensins in the different transgenic lines. rRNA bands stained using ethidium bromide indicate equal loading of total RNA in different lanes.