Table 4. Effects of DOX on mitochondrial transmembrane electric potential.

			ΔΨmax (-mV)						ADP Depolarization (mV)						Phosphorylative Lag Phase (sec)
Model	Substrate	Treatment	Heart		Liver		Kidney		Heart		Liver		Kidney		Heart		Liver		Kidney
			Mean	SE	Mean	SE	Mean	SE	Mean	SE	Mean	SE	Mean	SE	Mean	SE	Mean	SE	Mean	SE
Acute			n = 10		n = 9		n = 10		n = 10		n = 9		n = 10		n = 10		n = 9		n = 10
	Glutamate Malate	Saline	222.2	3.9	226.3	5.7	219.2	2.6	33.7	2.5	27.7	2.6	27.3	1.4	30.6	5.5	51.5	6.6	22.8	1.8
		DOX	224.3	5.4	226.4	4.4	217.8	2.9	38.1	3.2	27.7	2.9	29.0	1.0	25.5	3.2	43.3	4.9	25.8	2.8
	Succinate	Saline	223.7	2.9	231.8	5.0	222.9	2.7	42.2	1.4	33.3	1.8	31.7	0.8	41.6	6.8	68.0	8.4	27.4	2.8
		DOX	227.7	2.2	227.3	2.0	223.1	2.9	44.8	1.4	32.6	1.4	32.2	1.3	48.6	10.4	64.0	10.5	27.3	2.9
Sub-Chronic			n = 12		n = 11		n = 12		n = 12		n = 11		n = 12		n = 12		n = 11		n = 12
	Glutamate Malate	Saline	210.4	2.6	211.3	1.7	208.4	2.0	24.3	1.3	21.2	1.8	18.1	1.5	29.1	2.5	55.8	6.1	34.9	3.7
		DOX	208.1	3.1	207.4**	2.1	205.0**	1.7	24.2	0.9	22.4	1.4	18.9	1.9	32.2*	1.7	70.9*	11.5	38.9	3.5
	Succinate	Saline	214.9	2.6	217.0	1.6	211.1	3.4	27.0	1.6	26.2	1.7	19.7	2.0	38.4	3.1	64.4	6.2	38.2	3.6
		DOX	210.2**	3.0	216.5	2.4	209.2	4.1	27.0	1.7	26.5	1.9	19.2	2.2	43.5*	3.7	76.1	8.1	46.4	6.3

Data was collected with a TPP⁺-sensitive electrode (for details see Materials and Methods) where 225–250 nmol ADP were added to induce depolarization (phosphorylative cycle). Differences between treatment groups means within the same model were evaluated by matched pairs Student’s t test to exclude the variability related to mitochondrial isolation and electrode calibration but when assumptions were rejected the non-parametric Wilcoxon matched pairs test was applied (see Material and Methods for detailed information).

^*p≤0.05;

^**p≤0.01 vs saline group of the same model. SE – standard error.