Figure 2. Rescue of the ca5^cof mutant and acetazolamide treatment phenocopies the cof mutation in wild-type embryos.

(A, B) ca5^cof mutant embryos, (C–F) ca5^cof mutant embryos injected with 100 pg full-length wild-type ca5 (C, D) or 100 pg ca5^T839A mutant RNA (E, F). (I–L) Wild-type embryos at 2 dpf (I–K) and 3 dpf (L) treated with 5 mM AZA. Treatment of wild-type and cof heterozygous embryos at 2 dpf with 2.5 mM AZA.