Skip to main content
. 2012 Jun 22;7(6):e38910. doi: 10.1371/journal.pone.0038910

Figure 2. Temporal progression of changes in atrogin-1 and MuRF1 mRNA content and proteasome activity after unilateral hindlimb immobilization.

Figure 2

(A) Quantitation of mRNA content of gastrocnemius for atrogin-1 at various times after unilateral hindlimb immobilization, compared to contralateral non-casted control muscle. (B) Quantitation of mRNA for MuRF1 in gastrocnemius at various times after immobilization. All mRNA data were normalized to GAPDH and the WT control value was set at 1.0 arbitrary units (AU/GAPDH). (C) 20 S proteasome activity determined in immobilized and control muscle. *P<0.05, compared to contralateral non-casted control muscle of the same mouse. Values are means ± SEM; 7–9 mice per group.