Table 2. Effects of TPST-1 and TPST-2 knockout on neuronal populations, morphology and synaptic protein expression.
| Cell- or synapse-specific marker | Wildtype | TPST-1 knockout | TPST-2 knockout | Tpst DKO * |
| Photoreceptors | ||||
| PNA | Cone IPM and flat contacts with OFF-cone bipolar cells | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| WGA | IPM surrounding rod outer segments | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| VGLUT1 | All rod and cone terminals | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| SV2B | All rod and cone terminals | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| Syntaxin 3 | All rod and cone terminals | Present, but labeling may be reduced | Present, but labeling may be reduced | Present, but labeling may be reduced |
| Bipolar cells | ||||
| Synaptotagmin 2 | Type 2 OFF-cone bipolar cells and Type 6 ON-cone bipolar cells | Normal Type 2 OFF-cone bipolar cells; expanded Type 6 ON-cone bipolar cell plexus in IPL | Normal Type 2 OFF-cone bipolar cells; expanded Type 6 ON-cone bipolar cell plexus in IPL | Normal Type 2 OFF-cone bipolar cells; expanded Type 6 ON-cone bipolar cell plexus in IPL |
| PKC-α | Rod bipolar cells and terminals | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| Gγ13 | All rod and ON-cone bipolar cell bodies and terminals | Similar to wildtype | Similar to wildtype | Labeling present in rod bipolar and ON-cone bipolar cells, but reduced. |
| Goα | All ON-type bipolar cell bodies. Other processes in IPL | Similar to wildtype | Similar to wildtype | Labeling present in rod bipolar and ON-cone bipolar cells, and in processes in IPL but reduced. |
| Chx-10 | All bipolar cell nuclei | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| Islet-1 | All rod and ON-cone bipolar cell nuclei | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| VGLUT1 | All bipolar cell terminals | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| SV2B | All bipolar cell terminals | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| Syntaxin 3 | All bipolar cell terminals | Similar to wildtype | Similar to wildtype | Similar to wildtype, but labeling may be reduced |
| Horizontal cells | ||||
| Calbindin | Horizontal cells | Similar to wildtype | Similar to wildtype, but plexus in OPL reduced | Similar to wildtype, but plexus in OPL reduced |
| Amacrine cells | ||||
| Calretinin | Starburst amacrine cells, TH2 amacrine cells (and ganglion cells) | Similar to wildtype | Similar to wildtype | Starburst and TH2 amacrine cell plexes severely reduced. (Ganglion cells similar to wildtype) |
| VGLUT3 | Putative glutamatergic amacrine cells | Similar to wildtype | Process stratification similar to wildtype, but plexus reduced | VGLUT3 cells and plexus severely reduced |
| Tyrosine hydroxylase | Type 1 dopaminergic amacrine cells | Similar to wildtype | Process stratification similar to wildtype, but plexus reduced | Process stratification similar to wildtype, but plexus reduced |
| GAD-65 | GABAergic amacrine cells and processes in IPL | Similar to wildtype | Process stratification similar to wildtype, but labeling reduced | Process stratification similar to wildtype, but labeling strongly reduced |
| Ganglion cells | ||||
| MAP-1 | Ganglion cell dendrites and axons | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| Calretinin | Ganglion cell bodies and axons (and starburst and TH2 amacrine cells) | Ganglion cell labeling similar to wildtype | Ganglion cell labeling similar to wildtype | Ganglion cell labeling similar to wildtype (amacrine cell labeling reduced) |
| Glial cells | ||||
| Glutamate synthetase | Müller cells and astrocytes | Similar to wildtype | Similar to wildtype | Similar to wildtype |
| GFAP | Astrocytes and Müller cell endfeet | Similar to wildtype | Similar to wildtype | Similar to wildtype |
*
Tpst DKO data from Sherry et al., 2010.