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. 2012 Jun 22;7(6):e39729. doi: 10.1371/journal.pone.0039729

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Messina et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Total extract from secondary spermatocytes were immunoprecipitated with an anti-SAM68 antibody and analysed in Westeern blot with anti-MVH and anti-Sam68 antibodies. (B) Cellular extracts from spermatocytes (I sp.cytes), secondary spermatocytes (II sp.cytes) and round spermatids (sp.tids) were immunoprecipitated with an anti-SAM68 antibody and detected with anti-MVH antibody. Western blot analysis shows a specific interaction of the two proteins in secondary spermatocytes, while no signal is detected in other germ cell populations.