Figure 3. SCD5 activity blocks retinoic acid-induced differentiation of Neuro2a cells.

SCD5-expressing Neuro2a cells and empty plasmid-carrying controls (pCDNA4) were seeded in 60-mm dishes and incubated in 10%FBS DMEM. Twenty-four hours later, media was removed and cells were incubated with differentiation media (DMEM supplemented with 2%FBS and10 µM retinoic acid) for 48 h. Cells were fixed, stained with coomassie brilliant blue proliferation and photographed in a phase-contrast microscope (A). B, percentage (%) of cells bearing one or more neurites equal to or longer than cell body diameter.