Table 1.
New pharmacophore model | Old pharmacophore model | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Ligand | MOE stochastic search Born solvation MMFF94(S)[a] | MacroModel serial torsion search GB/SA solvation OPLS2005[b] | MOE stochastic search Born solvation MMFF94(S)[a] | MacroModel serial torsion search GB/SA solvation OPLS2005[b] | |||||||||
ΔE[c] | RMSD[d] | #c/#h[e] | ΔE[c] | RMSD[d] | #c/#h[e] | ΔE[c] | RMSD[d] | #c/#h[e] | ΔE[c] | RMSD[d] | #c/#h[e] | ||
Doxanthrine | Full | 0.5 | 0.60 | 2/1 | 0.0 | 0.11 | 14/14 | 0.0 | 0.12 | 2/2 | 0.0 | 0.11 | 14/6 |
SKF89626 | Full | 0.1 | 0.61 | 4/2 | 0.0 | 0.63 | 20/6 | 0.1 | 0.61 | 4/2 | 0.0 | 0.63 | 20/6 |
(−)-A86929 | Full | 0.0 | 0.54 | 10/6 | 0.0 | 0.52 | 73/58 | 0.0 | 0.54 | 10/10 | 0.5 | 0.12 | 73/36 |
A77636 | Full | 1.4 | 0.30 | 3/1 | 0.0 | 0.26 | 11/7 | 0.0 | 0.28 | 3/2 | 0.0 | 0.26 | 11/7 |
A70360 | Full | 1.5 | 0.65 | 7/1 | 13/0 | 0.0 | 0.67 | 7/5 | 2.1 | 0.67 | 13/5 | ||
Zelandopam | Full | 4/0 | 0.0 | 0.63 | 40/13 | 4/0 | 2.9 | 0.63 | 40/2 | ||||
SKF82958[f] | Full | 3.8 | 0.47 | 22/1 | 2.2 | 0.40 | 50/6 | 1.8 | 0.41 | 22/1 | 0.0 | 0.43 | 50/8 |
1 | Full | 1.6 | 0.59 | 2/1 | 1.6 | 0.61 | 6/2 | 0.0 | 0.22 | 2/2 | 0.0 | 0.13 | 6/4 |
2 | Full | 0.0 | 0.23 | 2/2 | 0.2 | 0.62 | 12/6 | 0.0 | 0.23 | 2/2 | 0.0 | 0.12 | 12/6 |
Dinapsoline | Full | 0.0 | 0.39 | 1/1 | 0.0 | 0.45 | 4/4 | 0.0 | 0.39 | 1/1 | 0.0 | 0.45 | 6/4 |
Dopamine | Full | 0.0 | 0.66 | 6/1 | 0.0 | 0.64 | 20/8 | 0.0 | 0.66 | 6/1 | 0.0 | 0.64 | 20/8 |
DHX | Full | 0.8 | 0.63 | 2/1 | 0.0 | 0.13 | 14/14 | 0.0 | 0.23 | 2/1 | 0.0 | 0.13 | 14/8 |
4[g] | Full | 0.0 | 0.55 | 2/2 | 0.0 | 0.56 | 16/8 | ||||||
4-OH-Dinapsoline[g] | Full | 0.0 | 0.36 | 1/1 | 0.0 | 0.46 | 8/5 | ||||||
6-Et-Dinapsoline[g] | Full | 0.0 | 0.37 | 6/3 | 0.0 | 0.46 | 16/16 | ||||||
A77641 | Partial | 3/0 | 11/0 | 3/0 | 2.8 | 0.67 | 11/2 | ||||||
A70108 | Partial | 6/0 | 0.0 | 0.56 | 11/7 | 0.0 | 0.71 | 6/3 | 0.0 | 0.72 | 11/7 | ||
3 | Partial | 0.0 | 0.62 | 2/2 | 0.9 | 0.61 | 8/4 | 2/0 | 8/0 | ||||
CY-208-243 | Partial | 3/0 | 1/0 | 3/0 | 1/0 | ||||||||
SKF38393 | Partial | 0.4 | 0.68 | 5/2 | 0.0 | 0.47 | 20/16 | 0.0 | 0.43 | 5/3 | 0.0 | 0.62 | 20/10 |
Apomorphine | Partial | 0.0 | 0.61 | 2/1 | 0.0 | 0.62 | 4/2 | 0.0 | 0.61 | 2/1 | 0.0 | 0.62 | 4/2 |
(+)-A86929 | inactive | 10/0 | 41/0 | 10/0 | 49/0 | ||||||||
Ro 21-7767[f] | Inactive | 4/0 | 8/0 | 4/0 | 8/0 | ||||||||
cis-DHX | Inactive | 5/0 | 12/0 | 2.7 | 0.62 | 5/1 | 3.4 | 0.62 | 12/4 | ||||
(−)-DHX | inactive | 0.8 | 0.74 | 2/1 | 8/0 | 0.8 | 0.74 | 2/1 | 8/0 | ||||
Rotigotine[f] | inactive | 1.3 | 0.73 | 186/5 | 1.9 | 0.72 | 486/13 | 3.6 | 0.69 | 186/2 | 486/0 | ||
(R)-NPA[f] | Inactive | 2.5 | 0.60 | 6/1 | 27/0 | 6/0 | 27/0 | ||||||
Sumanirole | Inactive | 5/0 | 4/0 | 5/0 | 4/0 | ||||||||
nPr-DHX[f] | inactive | 1.3 | 0.42 | 17/2 | 3.4 | 0.43 | 56/2 | 17/0 | 56/0 | ||||
Quinpirole | Inactive | 4/0 | 9/0 | 4/0 | 9/0 | ||||||||
5 | inactive | 2/0 | 8/0 |
The energy cutoff for conformations generated in MOE is 4 kcal mol−1.
The energy cutoff for conformations generated in MacroModel is 16.7 kJ mol−1 (∼4 kcal mol−1).
The lowest relative energy [kcal mol−1], with respect to the most stable conformer in the ensemble, for the conformers that fit the pharmacophore model.
Root of the mean square distance between the center of the pharmacophore features and their matching ligand annotation points.
#c: number of conformations generated for the respective method; #h: number of conformations that hit the pharmacophore model.
The amine is tertiary and therefore considered chiral, and two different configurations have been used in the modeling.
The compounds are new in this study and therefore were not screened against the previously published pharmacophore model.