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. 2012 Jun 22;7(6):e39320. doi: 10.1371/journal.pone.0039320

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Wu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A histogram of the number of samples versus average reference genes Ct value shows a normal distribution. It is possibly due to the nature of sample quality (both quantity and integrity) from LCM. Also this normal distribution supports viewpoint that ARG Ct value is a good indicator for sample quality.