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. 2012 Jun 22;7(6):e38535. doi: 10.1371/journal.pone.0038535

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Hu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Yeast strains defective in iron uptake (fet3fet4) and zinc uptake (zrt1ztr2) were transformed with pFL61 (empty vector) and pFL61-TcOPT3. Serial dilutions of yeast cells were dropped onto a low-zinc medium (LZM) supplemented with 50 µM ZnSO₄ (A) and a low-iron medium (LIM) supplemented with 10 µM FeCl₃ (B) assayed for growth on SD-ura plates. The entire experiment was performed twice.