Table 1. Primers used for PCR amplification of in TcOPT3 cDNAs, 5′ and 3′ RACE, qRealTime-PCR, and plasmid constructions in order of their first mention in “Materials and Methods”.

Purpose	Name	Sequence (5′-3′)
RACE PCR	TcOPT3-GSP-F	CAGTAACAGACAGGGACGATG
	TcOPT3-GSP-R	CAGCAGGTTGGCTCAGGGTA
	TcOPT3-5′R-1-R	ACCTGCTGAGCCGTGATGCTATTA
	TcOPT3-3′R-1-F	ACATCATCGTCCCTGTCTGTTACTG
	TcOPT3-5′R-2-R	CGACGATTGAGTGCGAAAAGAG
	TcOPT3-3′R-2-F	CTTCAAATCTCGCTCAAGTTTCTCT
Quantified-RealTime PCR	qRT-TcOPT3-F	CCGAGCAGCACGAGCGTTGT
	qRT-TcOPT3-R	GCGGTTGCGTGCGGTATGTG
	TcUBQ-F	GGAGCCCCGCTTGGAC
	TcUBQ-R	CGGCGAGGCGAGTGTA
in situ hybridization	TcOPT3-Probe-S	CCGGAATTCGGTTATCTCCTACGGCTTTG
	TcOPT3-Probe-A	CCCAAGCTTTAGGGCAAGCAGTGGTATCAA
Plasmid of GFP fusion	TcOPT3-EGFP-F	AAAAGTACTATGGACGCAGAGAAGGCTAACAAT
	TcOPT3-EGFP-R	TCCCCCGGGGAAAACAGGACAGCCTTTAACTCT
Plasmid of yeast transformation	TcOPT3-pFL61-F	ATAAGAATGCGGCCGCATGGACGCAGAGAAGGCTAA
	TcOPT3-pFL61-R	ATAAGAATGCGGCCGCAATCTTAGAAAACAGGACAGC

F, Forword; R, Reverse. S, Sense; A, Anti-sense.