Table II.

Levels of N²-ethylidene-dGuo (fmol/μmol dGuo) in granulocyte DNA before and after each dose of alcohol

	Subject	1	2	3	4	5	6	7	8	9	10
Dose 1	Baseline	7446	8467	15 290	9894	7196	9865	9095	8210	8881	7371
	2 h	7621	9815	16 750	13 690	6811	11 540	5580	8224	13 390	5659
	4 h	10 270	9968	23 140	15 740	9825	9595	6806	7863	—	4637
	6 h	7399	6983	13 000	15 740	9453	9301	7464	6733	7189	3155
	24 h	7397	8292	3403	2104	2158	3576	7978	4930	6008	5931
	48 h	10 400	6869	13 900	16 290	25 160	14 610	7244	5169	11 050	6707
	120 h	7290	5521	12 600	14 240	14 260	12 910	13 580	5769	6660	5546
Dose 2	Baseline	7991	12 550	9898	6623	6773	—a	3877	2178	—	5476
	2 h	8618	13 620	8158	7550	5859	—	3252	3224	—	19 250
	4 h	6208	15 530	11 580	7919	7188	—	3918	1795	—	4781
	6 h	3379	10 650	10 400	3758	6984	—	2835	1887	—	3053
	24 h	8886	12 780	6590	7521	6946	—	4654	4613	—	1361
	48 h	8076	8168	10 270	4923	10 820	—	3998	3168	—	2063
	120 h	20 440	15 460	6256	9313	9394	—	3951	4501	—	653
Dose 3	Baseline	—a	8697	7893	4358	7833	5988	4555	8498	9354	520
	2 h	4662	21 200	4689	4948	5625	3405	3123	14 710	7515	—
	4 h	6591	17 500	3040	5065	4895	4036	10 790	7885	4282	1181
	6 h	4565	9053	2276	5109	4141	1808	6235	4819	7133	3391
	24 h	11 960	9644	11 230	4488	22 360	6224	1102	3968	1025	813
	48 h	4774	5629	7552	13 070	40 630	11 560	3751	3543	3430	—
	120 h	3316	761	10 500	—	14 670	12 300	795	789	887	723
	168 h	3589	862	12 170	5481	5535	4643	913	1693	1076	9276
	1 week before dose 1	12 520	9331	8883	8980	9045	9656	9655	7409	7225	7708

Levels of N²-ethylidene-dGuo (as N²-ethyl-dGuo) were measured in granulocyte DNA from each subject. The last row reports participants’ adduct levels measured 1 week before the first dose was given. This is the point at which participants began to abstain from consumption of any alcoholic beverage other than the dose given for the study. ‘Baseline’ levels of N²-ethyl-dGuo were also measured 1 h before each dose was given. The rows that follow report the levels of N²-ethyl-dGuo at the various time points after consumption of the three increasing doses (from dose 1 to dose 3).

^aLevels of N²-ethyl-dGuo not quantified due to poor recovery.