Table III.

Levels of N²-ethylidene-dGuo (fmol/ μmol dGuo) in lymphocyte DNA before and after each dose of alcohol

	Subject	1	2	3	4	5	6	7	8	9	10
Dose 1	Baseline	8741	11 770	9858	12 400	1006	10 970	4923	3765	6518	5094
	2 h	11 310	8069	10 180	13 300	8870	10 440	4136	5200	7648	3991
	4 h	11 480	15 100	8702	11 820	7061	8479	5620	6206	4444	5397
	6 h	7783	6318	10 080	12 240	7575	9796	6248	3573	6385	6022
	24 h	13 550	5970	19 070	11 720	9227	15 910	5801	4356	5925	5200
	48 h	5762	7454	16 320	13 660	6394	12 510	5274	5696	13 900	3955
	120 h	4795	5128	10 970	13 760	7866	7389	3904	5513	4250	6231
Dose 2	Baseline	9576	13 110	16 960	13 650	9716	—a	2663	1902	—	1909
	2 h	10 700	10 380	14 810	8383	10 590	—	2157	2473	—	1688
	4 h	6787	6463	16 220	11 300	19 800	—	2203	1730	—	4728
	6 h	9750	8363	15 790	11 250	9410	—	1748	2507	—	3815
	24 h	5684	8569	11 800	10 270	7724	—	3080	3321	—	3782
	48 h	3008	2001	4605	6239	3506	—	2887	1458	—	2196
	120 h	8602	11 610	6072	4628	4377	—	3237	2411	—	2102
Dose 3	Baseline	—a	8063	7259	2939	5971	6164	4384	2801	932	4809
	2 h	7219	40 190	5145	4963	3986	4279	9334	10670	4576	—
	4 h	6811	2850	2909	2936	2226	5026	5802	3380	—	5864
	6 h	5600	8478	2966	4005	4595	1342	3609	2079	1798	11 660
	24 h	8144	4905	5198	4512	8579	7615	5079	1780	1254	804
	48 h	6539	2282	5598	4271	13 430	17 940	2645	2945	2698	—
	120 h	13 400	776	4537	5588	7126	1911	3071	909	660	4231
	168 h	4960	2965	9647	4708	1852	3510	1236	5389	3365	789
	1 week before dose 1	10 170	5431	8095	15 430	5878	9724	8192	5421	9582	4595

Levels of N²-ethylidene-dGuo (as N²-ethyl-dGuo) were measured in lymphocyte DNA from each subject. The last row reports participants’ adduct levels measured 1 week before the first dose was given. This is the point at which participants began to abstain from consumption of any alcoholic beverage other than the dose given for the study. ‘Baseline’ levels of N²-ethyl-dGuo were also measured 1 h before each dose was given. The rows that follow report the levels of N²-ethyl-dGuo at the various time points after consumption of the three increasing doses (from dose 1 to dose 3).

^aLevels of N²-ethyl-dGuo not quantified due to poor recovery.