Figure 5. Screening of TRAIL, TNF-α, FasL, cathepsin-D, and proteasome for their role in cell death induced by the combination of Smac mimetic and BCG-stimulated neutrophil conditioned medium.

All Western blots were performed using cells treated for 24 h as indicated. (A and B) Western blotting for caspase-8 Asp-391/374. (Note the p26 band; TRAIL is not efficient in generating p26, which indicates that the p26, generated by Smac mimetic in combination with BCG-stimulated neutrophil conditioned medium in Fig. 4A, is not a result of TRAIL.) (C) Quantitative ELISA to evaluate autocrine secretion of TNF-α. (D) Quantitative ELISA to evaluate autocrine secretion of TRAIL. *The conditioned media were diluted 1:1 with fresh complete MEM.