A, representative Western blots of phosphorylated S6K1 (Thr-389) and total S6K1 in homogenates of mice placenta from control and fADN group at E18.5. C, summary of Western blot data from control (C, n= 9) and fADN (A, n= 7) infused mice. After normalization to β-actin, the mean density of control samples was assigned an arbitrary value of 1. Individual C and A density values were expressed relative to this mean. Values are given as means + SEM. *P < 0.05 versus control; Student's unpaired t test.