Figure 2. MFG-E8-deficient DC exhibit increased uptake of necrotic tumor cells.

PKH26-labeled EL4 cells were treated with CDDP or γ-irradiation (IR) (30 Gy) to induce apoptotic cell death, or treated with CDDP and zVAD-fms to induce necrosis. The apoptotic or necrotic cells were incubated with BMDCs from wild-type (WT) or MFG-E8-deficient (MFG-E8-KO) mice for 2 h. In some instances, necrotic cells were treated with the RIP1 inhibitor Necrostatin-1 or DMSO for 48 h before co-culture with DC. The uptake of EL4 cells by CD11c⁺BMDCs was quantified by flow cytometry. Representative data (A) and statistical analysis from two experiments (B) are shown. The percentage of PKH26⁺CD11c⁺ and PKH26⁻CD11c⁺ populations was shown in right-upper and right-bottom quadrant of each data, respectively. Results are representative of two independent experiments. * p<0.05, ns: not significant.