Fig. 3.

Overexpression of miR-17-92 promotes cell-cycle progression of effector CD8⁺ T cells. (A) Splenocytes from Thy1.1⁻ P14 mice were transduced with MIT or miR-17-92-MIT and transferred to C57BL/6 mice, which were subsequently infected with LCMV Arm and pulse-labeled with BrdU on day 6 or 7 p.i. (B Left) Gating of transduced (Thy1.1⁺; red) and nontransduced (Thy1.1⁻; blue) P14 cells. (Right) Frequencies of BrdU⁺ cells within transduced and nontransduced P14 T cells in the spleens of each group (MIT or miR-17-92-MIT) on days 6 and 7 p.i. Each line represents data from one individual mouse. Results are representative of at least two experiments with n ≥ 3. Paired Student’s t test was used. *P < 0.05, **P < 0.01, ***P < 0.001.