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. 2012 Jun 4;109(25):9905–9910. doi: 10.1073/pnas.1115063109

Fig. 2.

Fig. 2.

ARHGEF11 localization to TJs is ZO-1–dependent. (A) Frozen sections of mouse mammary gland were coimmunostained for ARHGEF11 and ERM (Top), ARHGEF11 and ZO-1 (Middle), or ARHGEF11 and E-cadherin (Bottom). Asterisks indicate the lumen of the mammary ducts. (B) Expression and localization of ZO-1 and ARHGEF11 in EpH4 and ZO1KO-EpH4 cells was analyzed by immunoblotting and immunostaining. (C) Depletion of ARHGEF11 did not prevent ZO-1 from localizing to TJs. Control or ARHGEF11-specific siRNA was introduced into EpH4 cells, then the expression and localization of ZO-1 and ARHGEF11 were examined. Blue indicates DAPI staining. (Scale bars: 10 μm.)