Combining our structural results with values for bending modulus κ and spontaneous curvature c0 allows to estimate the bending energy of a stalk: (A) Bending energy term κ(Σ1 + c0Σ2) for the case of DOPC (κ = 9 kBT, c0 = -0.0115 Å-1) and an equimolar DOPC/DOPE mixture (κ = 9 kBT, c0 = -0.024 Å-1) as a function of the isodensity value ρiso The values of κ and c0 were obtained from (16), in case of the lipid mixture, molar fraction-weighted values are used. (B) The same energy for a fixed isosurface (DOPC/DOPE 1∶1, ρiso = 0.34) as a function of κ and c0. If c0 becomes slightly more negative upon dehydration, as explained in the main text, κ(Σ1 + c0Σ2) may approach negative values.