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. 2012 Jun 4;109(25):9935–9940. doi: 10.1073/pnas.1119459109

Fig. 5.

Fig. 5.

Reduced intracellular TNF of Siglec-13–transfected RAW264.7 cells in response to bacterial pathogen infection; 500,000 cells semistably transfected with Siglec-13 or vector only and selected with G418 for 3 wk were seeded in a 12-well plate. The next day, 2 h before infection, cells were washed three times with HBSS, and regular culture medium without G418 added. Cells were infected with bacterial pathogens at an appropriate MOI for 1 h at 37 °C. The BD Fixation and Permeabilization Solution Kit (555028) with APC rat anti-mouse TNF-α was used to detect the intracellular TNF level using the recommended protocol. Cells without bacterial infection were used as controls. Cells transfected with pcDNA3.1 vector were used as a control. (A) Cells infected by E. coli K1 RS218 (StrR) at MOI = 0.6. (B) Cells infected by GBS A909 (serotype Ia) at MOI = 0.1.