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. 2012 Jun 1;109(25):9672–9673. doi: 10.1073/pnas.1206735109

Fig. 1.

Fig. 1.

Identification of O-GalNAc–containing glycoproteins modified by specific ppGalNAcT isoforms. SimpleCells that are devoid of C1GalT1 activity were generated by zinc finger nuclease mutagenesis in liver HepG2 cells by ablating the chaperone Cosmc. Following trypsin digestion, those glycopeptides that have an O-GalNAc were bound by the GalNAc-specific lectin, Vicia villosa agglutinin (VVA), and analyzed by MS. O-GalNAc peptides from HepG2 SimpleCells and HepG2 SimpleCells devoid of GalNAc-T2 activity (T2−/−) allowed for the identification of proteins and peptides that were the exclusively modified by GalNAc-T2 (blue line) such as ApoC-III.