Figure 2. Tumorigenesis in KO mice following DEN/PB treatment is associated with greater injury, fibrosis and regeneration.

A. Representative tumor-bearing KO livers show increased parenchymal cell death (TUNEL), greater stellate cell activation (α-SMA) and increased parenchymal cell proliferation (PCNA) as compared to WT. B. Increased hepatic fibrosis (blue) in tumor-bearing KO livers is evident by Masson Trichrome staining as compared to control. C. PCNA- and TUNEL-positive cells were counted in 4 random sections of 5 representative KO and WT livers. A significant increase in both PCNA and TUNEL positive parenchymal cells was evident in KO livers as compared to WT after DEN/PB treatment.