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. 2012 Jun 25;7(6):e39095. doi: 10.1371/journal.pone.0039095

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Deng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Western Blotting analysis of Chk1, p-Chk1(S345), p-Cdc2(Y15) and Cdc2. Actin shows protein loading controls. B, Relative mitotic index at different time points after γ-ray irradiation. Error bars represent standard deviations. Arrows indicated the relative mitotic indices in HONE1-LMP1 cells transfected with control plasmids (pEGFP) significantly higher (P<0.05) than HONE1-LMP1 cells transfected with Chk1-expressing plasmids (pEGFP-Chk1). C, Frequencies of chromatid breaks before or after γ-irradiation. Error bars represent standard deviations. Stars indicated significant differences (P<0.05) between the indicated datum pairs.