Figure 2. Effects of saline and ANGII treatments on SCT promoter activity and gene expression in N-42 cells.

(A) Osmolality of culture medium after treatment of different concentrations of saline (0, 50, 100, 200, 300, 400 mM) for 8 h in N-42 cells. (B) Percentage of cells surviving after saline treatments with medium of various concentrations (0, 50, 100, 150, 200, 300, 400 mM) for 8 h in N-42 cells. (C) Cells were transfected with mSCTP (2.0 µg) for 2 d and treated with various saline concentrations (25, 50, 100 mM) for different times (2, 4, 8 h) or ANGII (10⁻¹⁰ to 10⁻⁷ M) for 8 h. Data represent the mean ± SEM of three experiments performed in triplicates. (D and E) Cells were treated with various saline concentrations (25, 50, 100 mM) for different times (2, 4, 8 h) and RNA was extracted afterwards. The mRNA levels of mouse SCT (D) and S14 (E) were normalized with mouse GAPDH levels. Data represent the mean ± SEM of three experiments performed in duplicates.*p<0.05; **p<0.001, compared with the respective control.