Figure 4. Effects of water deprivation, saline dinking and central ANGII administration on mouse ERRα expressions in mouse brain.

Mice were water deprived, provided with hypertonic saline (2%) for 1 or 5 d, or centrally injected with ANGII peptide. The mRNA levels of mouse ERRα in (A) the whole mouse brain or (C) the isolated osmosentitive brain regions SFO, OVLT, MnPO and PVN, were measured by real-time PCR were normalized with mouse GAPDH levels. Data are expressed as the mean ± SEM (n = 10/group). *p<0.05; **p<0.001, compared with control. (B) Immunohistochemical staining showing ERRα immunoreactivities in the SFO, MnPO, OVLT and PVN of the WT mouse brain. Negative control was done by using 1× PBS instead of the primary anti-ERRα antibody. Bars, 6 µm.