Table 1. Nucleotide sequences of siRNA oligos, EMSA oligos, real-time PCR and mutation analysis (mutated nucleotides are underlined) primers.
Primer | Sequence (5′- 3″) |
siERRα-S1-S | GATCCCTTTGCCTTTCCCGGGCCCTTCAAGAGAGGGCCCGGGAAAGGCAAAGGGA |
siERRα-S1-AS | AGCTTCCCTTTGCCTTTCCCGGGCCCTCTCTTGAAGGGCCCGGGAAAGGCAAAGG |
siERRα-S2-S | GATCCTGCTGGACCTCTGGCTCTATTCAAGAGATACAGCCAGAGGTCCAGCAGGA |
siERRα-S2-AS | AGCTTCCTGCTGGACCTCTGGCTGTATCTCTTGAATACAGCCAGAGGTCCAGCAG |
GS-ERRα-S | GCCCCTGACCTTCCCGG |
GS-ERRα-AS | CCGGGAAGGTCAGGGC |
SCTP-F | ACTACTCCTCTAATCTCCCC |
SCTP-R | GACAAATGCTGCAACTTCAG |
GAPDH-F | TGTGTCCGTCGTGGATCTGA |
GAPDH-R | CCTGCTTCACCACCTTCTTGAT |
SCT-F | GGTGGAGGGCCTCTATCTTC |
SCT-R | CCAGGCGCTTATAATGGTGT |
ERRα-F | TTCGGCGACTGCAAGCTC |
ERRα-R | CACAGCCTCAGCATCTTCAATG |
S14-F | CAGGACCAAGACCCCTGGA |
S14-R | ATCTTCATCCCAGAGCGAGC |
M1 | GAGGGACAGGAGACAGCCGGACGACAG |
M2 | GGTGGGCGGCCCCAGCCTTCCTGCAAT |
M3 | TGGGCGGCCTGATTCTTCCTGCAATCA |
M4 | GGTGCAGCATTTAGTACACCCAGAGCC |