Figure 3. TC11 inhibits tumor cell growth and induces apoptosis in vivo.

KMS34 (1.2×10⁷ cells) were injected intraperitoneally into lcr/scid mice and plasmacytoma was established. When the size of the tumor had reached 50 mm³ (day 1), 100 µL of TC11 (20 mg/kg) or vehicle alone (10% DMSO, 1% Tween 80-saline) was injected intraperitoneally into a mouse twice with a 3-day interval (i.e., injections were done on days 1, 2, 4, 5, 7, 8, 10, 11, 13 and 14). (A) The width and length of the plasmacytoma were measured and tumor volume was calculated (n = 7). (B) Sections were stained with hematoxylin and eosin (HE). Cells with aggregated chromatin are indicated by white arrows. (C) Apoptotic cell death was detected by immunohistochemical staining with anti-single-stranded DNA antibody. Apoptotic cells are indicated by white arrows. The plot on the right side shows that the difference in the number of ssDNA-positive cells between the vehicle and TC11 groups was statistically significant (Student’s t-test, P<0.002). For this plot, the number of ss DNA-positive cells was counted in two fields per tumor in five tumors (10 fields in all) in each group.