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. 2012 Jun 25;7(6):e38878. doi: 10.1371/journal.pone.0038878

Figure 5. Monomeric NPM interacts with TC11.

Figure 5

(A) The domain structure of NPM and the amino acid sequence predicted by NPM clone 1–183, which was identified as encoding a binding protein of TC11 by mRNA display using a cDNA library prepared from KMS34 cells. The underlined part indicates the region (1–183 a. a.) identified by mRNA display selection. The sequence indicated by the solid line corresponds to that encoded by the ORF of NPM cDNA. (B) Recombinant NPM1–183 was expressed in E. coli and fractionated into monomeric and oligomeric forms. Then, each fraction was subjected to 10% SDS-PAGE followed by CBB staining (left). A representative biosensorgram of NPM binding on SA sensor chips with immobilized biotinylated-TC11 is shown. The K D values were determined (right).