Figure 4.

Costimulation restores the inhibition of AP-1 activation by Dex. Nuclear extracts were prepared from Dex-treated and control naive CD4+ T cells stimulated with anti-CD3, anti-CD3 plus IL-2, or anti-CD3 plus anti-CD28 and analyzed by electrophoretic mobility shift assay (EMSA) using 32P-labeled oligonucleotide probes corresponding to (a) AP-1, (b) NF-AT, or (c) NF-κB DNA binding sites. As a control, the competition (Comp) for DNA binding with excess oligonucleotide was assessed. Results from a representative experiment are shown.