Figure 9.
Dex alters IL-2–induced activation of STAT5. (a) Dex-treated and control naive CD4+ T cells were stimulated with IL-2 for 20 minutes, and cell extracts were prepared and assessed for STAT5 DNA binding using EMSA. (b) Dex-treated and control naive CD4+ T cells stimulated with anti-CD3 or anti-CD3 plus IL-2 for 4 or 24 hours were harvested, washed, rested, and stimulated with IL-2 for 20 minutes. STAT5 DNA binding was assessed in the cell extracts by EMSA.