Table 1. Melting curve analysis of VTEC target plasmids and pure cultures of reference strains using the SYBR®Green Real-time simplex or multiplex reactions on an AB 7500, Biorad iQ5 and Roche LightCycler 480 PCR thermal cycler (STP: standard plasmid).

AB7500				Roche LC480			Biorad iQ5
DNA	stx or eae	stx/eae	stx/eae/aggR	stx or eae	stx/eae	stx/eae/aggR	stx or eae	stx/eae	stx/eae/aggR
STP ( stx 1)	77.3±0	N/A	N/A	80.65±0.057	N/A	N/A	81.67±0.289	N/A	N/A
STP (stx2)	78.72±0.606	N/A	N/A	83.14±0.063	N/A	N/A	83.50±0.001	N/A	N/A
STP (eae)	75.70±0.001	N/A	N/A	80.11±0.02	N/A	N/A	79.50±0.001	N/A	N/A
Genomic DNA	77.15±0.446or75.63±0.265	76.08±0.809	78.53±0.953	82.28±0.760or79.30±0.078	82.22±0.05078.24±0.010	81.23±0.05979.59±0.03582.88±0.355	81.33±0.258 or 80.00±0.001	81.70±0.274 or 79.5±0.289	81.58±0.20479.83±0.25883.00±0.001