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. 2004 Feb 15;113(4):609–618. doi: 10.1172/JCI19097

Figure 5.

Figure 5

The antimitogenic effect of APOE requires Cox-2. Murine aortic SMCs were serum starved and treated with 10% FBS in the absence (control) or presence of 2 μM APOE. Cells were also treated with 0.1–1 μM nimesulide or 0.01–5 mM SC560. All the cells were incubated for 48 hours in the presence of BrdU. (a) The conditioned media was collected and assayed for 6-keto-PGF1α. Results show mean ± SEM, n = 2, *P < 0.005 as compared with cells treated with 10% FBS (control) and 2 μM APOE. (b) BrdU incorporation into nuclei was determined by immunofluorescence microscopy. Results show mean ± SEM, n = 2, *P < 0.005 as compared with cells treated with 10% FBS (control). (c) Quiescent murine aortic SMCs were treated with 10% FBS for 24 hours in the absence (control) or presence of APOE and 0.5 μM nimesulide. Cells were collected, lysed, and analyzed by immunoblotting for cyclin A and cdk4 (loading control).