Fig. 1.
a–c Normalized SPR sensorgrams showing benzamidine (a) and CC 00813 (b) binding to immobilized trypsin. Both compounds were injected as an eight-membered twofold dilution series (including ‘zero buffer blank’) with a top concentration of 160 μM for benzamidine and 256 μM for CC 00813. Diagram in c show fits of the binding responses at equilibrium (t = 50–55 s, plotted against compound concentration) to a 1:1 steady state affinity model. As CC00813 failed to reach a maximal binding response (Rmax) at the top injected concentration (256 μM), its affinity (KD = 466 μM) was estimated using Rmax values determined from a benzamidine binding fit