Table 1.

Activation rate and equilibrium constants for wt AChRs

Agonists	kon	koff	Kd	Concentrations
	mM−1s−1	s−1	M	mM
DMP	1,383	2,838	2.1 E-3	1, 2, 3
DMT	1,991	3,194	1.6 E-3	1, 2, 3
Nic	2,484	2,501	1 E-3	1, 2, 3
TMA	6,770	5,515	0.81 E-3	0.3, 0.5, 0.7
CCh	17,500	7,178	0.41 E-3	0.1, 0.2, 0.3, 0.5
ACh	106,108	17,614	0.16 E-3	0.03, 0.1, 0.3

Concentrations are those used for the estimation of K_d. In all cases, the SEM of the rate constants were <10% of the optimal values shown. k_on, k_off, agonist association and dissociation rate constants to resting receptors; K_d (=k_off/k_on), the LA agonist equilibrium dissociation constant to resting receptors; J_d, the HA agonist equilibrium dissociation constant to open-channel (R*) receptors.