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. 2012 Jul;140(1):69–82. doi: 10.1085/jgp.201210776

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© 2012 Brauser et al.

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Figure 8. — Dependence of the rate constants (Eq. 1) of fast blocking on the concentration of enrofloxacin given at the trans side (A and B) or cis side (C and D). k_OB (A and C) is the rate constant of blocking (binding of antibiotic) and k_BO (B and D) of unblocking (dissociation of the antibiotic from the protein). Black squares: +50 mV; red circles: +75 mV; green upright triangles: +85 mV; blue inverted triangles: +100 mV. Dashed lines in A and C are linear fits through the origin. For the sake of clarity, the datasets from LPS and DPhPC membranes were pooled, and the symbols show mean and standard error. A separate presentation for both membrane types is given in Fig. 9 and Table 1, where the number of experiments is also given.