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. 2012 Jul;140(1):41–53. doi: 10.1085/jgp.201210796

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© 2012 Telezhkin et al.

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Figure 2. — Single M-type Kv7.2/7.3 channel activity in excised inside-out membrane patches activated by exogenous DiC₈-PI(4,5)P₂. (A) Examples of single-channel recordings showing effects of sequential increases of DiC₈-PI(4,5)P₂ concentrations applied to the inner leaflet of excised inside-out patches from K2/3-CHO cells stably expressing M-type Kv7.2/7.3 channels. Holding potential is 0 mV. C, closed channel current level; O, open channel current level. (B) As in A but with patches excised from HM1-CHO cells transiently transfected with a concatenated Kv7.2/7.3 channel cDNA. O1 and O2, open channel current levels for 1 and 2 channels. (C) Mean (±SEM) for P_open plotted against DiC₈-PI(4,5)P₂ concentration. Open circles represent K2/3-CHO cells stably transfected with coexpressed Kv7.2 and Kv7.3 channels. Filled circles represent HM1-CHO cells transiently transfected with a concatenated Kv7.2/7.3 cDNA. The solid line represents a least-squares fit of the open circle data to a two-component Hill equation (see Materials and methods) giving the parameter values indicated in the text. (inset) The mean (±SEM) channel P_open plotted against DiC₈-PI(4,5)P₂ concentration for patches where analysis indicated that only a single active channel was present (n = 5–14 patches at each concentration) is shown. The data were fitted with a two-component Hill equation with parameter values as given in the text.