Figure 1.

Association of PP5 with ATM and hRad17. (A) Coimmunoprecipitation of endogenous PP5 and hRad17. HEK 293T cells were left untreated or treated with 100 ng/mL NCS for 30 min prior to lysis. Preimmune serum (PI) was used in control immunoprecipitation as indicated. (B) DNA-damage-induced association of ectopically expressed PP5^WT and ATM in HEK 293T cells was determined by α-Flag immunoprecipitation followed by α-HA immunoblotting (lower panel). (C) Association of endogenous ATM with PP5 was determined by immunoprecipitation assays with α-ATM monoclonal antibody with or without treatment of NCS as indicated. Normal mouse IgG (IgG) was used in the control immunoprecipitation.