Figure 2.

The S609A mutation inhibits activation of Src family kinases. (a) 1b9 or S609A cells were stimulated by ristocetin in the presence or absence of VWF, then solubilized and subjected to Western blot analysis with anti-Src and anti-phospho-Src family (pTyr416) antibody, respectively. The immunoblot is representative of 3 different experiments; (b) Quantitative data from 3 different experiments (mean ± SD) are demonstrated. The relative activation index of Src equals arbitrary quantitation of phospho-Src (pTyr416)/arbitrary quantitation of total Src.