Figure 1.
Circadian conidiation rhythms of the ppp-1RIP strain show advanced phase and short period phenotypes. (A) Phosphatase activity assay showing the reduction of PP1 activity in the ppp-1RIP strain. Cultures were cultured and harvested in LL. I-2: assay containing 100 U of inhibitor-2. Error bars, standard deviations. (B) Race tube assay showing the circadian conidiation rhythms of the wild-type and the ppp-1RIP strains in DD. The black lines mark the growth fronts at 24-h intervals. The calculated period length and phase are shown below. (SD) Standard deviation. (C) Race tube assay in LD cycles. To show the difference of the position of the conidiation peaks between the two strains, the image of the ppp-1RIP mutant was enlarged so that it showed similar daily growth distance as the wild-type strain.