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. 2004 Feb 1;18(3):255–260. doi: 10.1101/gad.1152604

Figure 2.

Figure 2.

Circadian rhythm of FRQ expression and its stability in the wild-type (•) and ppp-1RIP(○) strains. (A) Western blot analysis showing the circadian oscillation of FRQ in DD. Densitometric analysis of the Western blot results is shown at bottom. Two independent experiments were performed and similar results were obtained (see Supplemental Fig. 1). (B) Western blot analysis showing that FRQ is less stable in the ppp-1RIP strains after the addition of CHX (10 μg/mL). Cultures were grown in LL. Densitometric analysis of the Western blot results from seven independent experiments is shown at bottom. Error bars, standard deviations. (*) P values for 3, 6, 9, and 12 h are 0.02, 0.003, 0.001, and 0.0009, respectively.