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. 2004 Feb 1;18(3):278–289. doi: 10.1101/gad.1152204

Figure 1.

Figure 1.

p160MBP interacts with GST-PGC1(200-400) and suppresses PGC-1α-mediated transcription. C2C12 muscle cell nuclear extracts were incubated with bacterially expressed GST fusion proteins on glutathione sepharose beads. PGC(1-200) is GST-PGC1α(1-200) and PGC(200-400) is GST-PGC1α(200-400). The interacting proteins were run on an SDS-PAGE gel and (A) silver stained or (B) Western blotted with antibodies against p160MBP. (C) p160MBP or p67MBP was cotransfected with Gal4-PGC1α or Gal4-PGC1(Δ170-350) and a UAS-luciferase reporter in HIB1B cells. Luciferase activity was measured after 24 h. (Asterisks) P < 0.001, paired t-test. (D) PPARγ and RXRα with or without PGC-1α were cotransfected with a DR-1-luciferase reporter into HIB1B cells. p160MBP or p67MBP were also added and luciferase levels were measured after 24 h. All luciferase assays were normalized for transfection efficiencies using β-galactosidase. (Asterisks) P < 0.001, paired t-test.