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. 2004 Feb 1;18(3):278–289. doi: 10.1101/gad.1152204

Figure 4.

Figure 4.

p160MBP suppresses PGC-1α's biological effects in muscle cells. C2C12 myoblasts were coinfected with adenoviral PGC1α and p160MBP. Adenoviral GFP was used in the control infections. After 48 h, (A) total mitochondrial oxygen consumption and (B) uncoupled respiration were measured in live muscle cells. Uncoupled respiration represents the fraction of mitochondrial respiration that is not coupled to ATP production and was attained through addition of the ATP-synthase inhibitor oligomycin. (Asterisks) P < 0.05, repeat ANOVA, a posteriori Tukey test. (C) Same as in A and B, except instead of using cells for oxygen consumption assays, RNA was harvested and Northern blots were performed. (D) C2C12 myoblasts were coinfected with adenoviral PGC1α and p160MBP in serum-free media. TNFα, IL-1α, and IL-1β were added at the time of infection and cells were harvested after 24 h. Real-time PCR was used to quantitate RNA levels. (Asterisks) P < 0.05, paired t-test; (n.s.) not statistically significant, paired t-test.