Figure 2.

The effect of Ecklonia cava enzymatic extract on the anagen induction in C57BL/6 mice. After shaving, the back skins were treated with 0.5% E. cava enzymatic extract, vehicle and 5% minoxidil every day for 33 days. (A) The back skins were photographed at 1, 7, 13, 20, 26 and 33 days after depilation; (B) On day 26, the quantitative assessment of anagen induction analyzed via dotmatrix planimetry was performed. The transparency was put on a photo of a mouse to mark the areas that were in different stages (pink = telogen, anagen = black). Afterward a dotmatrix (sheet with a uniform defined dot pattern) was placed under the marked foil to calculate the percentages of the regions of interest by counting the dots. The percentage of anagen induction was calculated by the equation [(black skin/total skin) × 100]. Data are presented as the mean ± SE (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. vehicle treated control.