Figure 9.

Effect of ovatodiolide on tyrosinase activity in B16F10 cells. Enzyme assay was performed as described in the “Experimental Section”. Briefly, B16F10 melanoma cells were stimulated with α-MSH (100 nM) for 24 h, and the cellular tyrosinase activity was assayed after treatment with ovatodiolide (0.02, 0.08 and 0.16 mg/mL) or arbutin (0.545 mg/mL) for another 24 h. Results are represented as percentages of control, and the data are mean ± SD for three separate experiments. Values are significantly different by comparison with control. ^*** p < 0.001.