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. 2004 Feb 1;18(3):306–319. doi: 10.1101/gad.1162404

Figure 7.

Figure 7.

p44 effects on cell size and cell number. (A) Lymphocytes. Relative sizes of gated subpopulations of a total of 20,000 spleen cells determined in forward scatter mode by FACS. Histograms of cell counts in populations of activated lymphocytes (top), large granulated cells (middle), and immature lymphocytes (bottom) are color-coded by genotype: red, NT splenocytes; green, hemizygous splenocytes; blue, homozygous splenocytes. (B) Testis. Relative mass of spermatocytes at 3 wk of age, as determined by image analysis. See Materials and Methods for details. (C) Liver. Relative area of cells from adult liver, as determined by image analysis. See Materials and Methods for details. (D) Embryonic cell proliferation. Assays were performed at successive passages with cells derived from E12.5-14 embryos. Shown is a plot of average cell counts over the 10-d period for cultures derived from NT (red), hemizygous (green), and homozygous (blue) embryos at P0, P1, P2, and P3. P0 were primary cells that had not been cultured for any length of time prior to the assay. Shown is one set of assays; identical results were obtained with cells from at least two different embryos of each genotype. Hemizygous cells consistently proliferated at a higher rate than NT cells at P1. See the discussion of the IGF-R in the text for an explanation.