TABLE 6.
Inhibitory effect of oseltamivir on [3H]GlySar uptake in P. pastoris cells expressing different mammalian species of Pept1 gene
Parameter estimates were expressed as means ± S.E. (n = 6). The data were fitted to eq. 2 using a weighting factor of 1/Y. GlySar was studied at 5.0 μM, whereas oseltamivir concentrations ranged from 0.5 to 200 mM. GlySar uptakes in vector alone were reduced by 10% or less over the oseltamivir concentrations studied.
| Parameter | Yeast Transformant |
||
|---|---|---|---|
| pPIC3.5K-hPept1 | pPIC3.5K-mPept1 | pPIC3.5K-rPept1 | |
| IC50, mM | 27.4 ± 2.3 | 10.7 ± 0.5 | 18.3 ± 0.9 |
| N | 0.90 ± 0.06 | 1.07 ± 0.03 | 1.11 ± 0.04 |
| r2 | 0.888 | 0.978 | 0.963 |