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. 2004 Feb 1;18(3):320–332. doi: 10.1101/gad.1162204

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Copyright © 2004, Cold Spring Harbor Laboratory Press

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Figure 7. — Interaction between Ovca1 and p53 mutations for tumor formation. (A) H&E-stained section of a representative mammary gland adenosquamous carcinoma was found in two Ovca1-2^+/- p53^+/- mice at 38 and 78 wk of age. There are keratinized well-differentiated squamous cells surrounded by the glandular elements of the mammary gland. Scale bar, 100 μm. (B) H&E-stained section shows a bronchiolo-alveolar carcinoma with a papillary growing pattern in an Ovca1-2^+/- p53^+/- male mouse at 80 wk of age. Scale bar, 100 μm. (C) Reproductive tract from an Ovca1-2^+/- p53^-/- female mouse at 33 wk of age. One ovary (arrow) shown at the right is enlarged and hemorrhagic. H&E-stained sections of ovaries shown in C with normal histology (D) and a hemangiosarcoma immunostained with CD34 (brown; E). (F) H&E-stained section of a brain tumor (T) showing a transition from the cerebellar granular layer to a neoplastic appearance reminiscent of human medulloblastoma, replacing the cerebellar folia. (G) Southern blot analysis of p53 and Ovca1 in tumors from Ovca1-2^+/- p53^+/- and p53^+/- mice. Tail DNA from wild-type (+/+), cis Ovca1-2^+/- p53^+/- (+/-), and Ovca1-2^-/- p53^-/- (-/-) mice. Genomic DNA from individual tumors, including trans Ovca1-2^+/- p53^+/-, cis Ovca1-2^+/- p53^+/-, and p53^+/-. (m) Mutant allele; (+) wild-type allele; (ψ) p53 pseudogene. One cis Ovca1-2^+/- p53^+/- tumor shows LOH of the p53 locus and an additional minor band (arrow), suggesting a rearrangement of the Ovca1 locus in a subset of cells in the tumor.