TABLE 1.
Effect of GLP-2 on basal biliary and urinary secretory function and on serum and tissue levels of DNP-SG
Basal bile and urine were collected for 10 min, and corresponding flows were estimated gravimetrically. Serum and tissue contents of DNP-SG were assessed by high-performance liquid chromatography 5 min after administration of CDNB (30 μmol/kg b.wt.). Data are means ± S.D.
| Parameters | C (n = 4) | GLP2 (n = 4) | |
|---|---|---|---|
| Basal conditions | Bile flow (ml · min−1 · g liver−1) | 1.61 ± 0.15 | 1.69 ± 0.22 |
| Urinary flow (ml · min−1 · g kidney−1) | 8.7 ± 0.5 | 8.2 ± 0.3 | |
| Biliary excretion of total glutathione (nmol · min−1 · g liver−1) | 3.42 ± 0.35 | 3.36 ± 0.29 | |
| Biliary excretion of oxidized glutathione (nmol · min−1 · g liver−1) | 1.22 ± 0.30 | 1.18 ± 0.25 | |
| Biliary excretion of bilirubin (nmol · min−1 · g liver−1) | 0.132 ± 0.005 | 0.136 ± 0.004 | |
| Serum concentration of DNP-SG (mM) | 0.008 ± 0.001 | 0.009 ± 0.002 | |
| After CDNB administration | Liver content of DNP-SG (nmol/g liver) | 7.1 ± 1.1 | 4.5 ± 0.3* |
| Intestinal content of DNP-SG (nmol/g intestine) | 2.8 ± 1.0 | 9.8 ± 5.2* | |
| Renal content of DNP-SG (nmol/g kidney) | 3.4 ± 0.7 | 3.1 ± 0.6 |
*
Significantly different from control (C), P < 0.05.